7. Peotone Glucose Yeast extract Medium PGY (蛋白腖、酵母膏、葡萄糖培養基)
Peptone(蛋白腖) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸餾水) 1L
8. Glycerol Agar (甘油瓊脂)
Peptone (蛋白腖) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自來水) 1000ml
Agar (瓊脂) 15g pH 7.0-7.2
9. Rhizobium medium (根瘤菌培養基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (瓊脂) 15g
Distilled water (蒸餾水) 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的製法:取土壤50克,加水200毫升,15磅蒸煮1小時,經濾紙過濾後加水補足到200毫升。)
適用範圍:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫雲英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌
10. Mannitol Agar (甘露醇瓊脂)
Yeast extract (酵母膏) 5g Peptone (蛋白腖) 3g
Mannitol (甘露醇) 25g Agar (瓊脂) 15g
Distilled water (蒸餾水) 1000ml
11. Glucose Asparagine (葡萄糖、天門冬素瓊脂)
Glucose (葡萄糖) 10g Asparagine (天門冬素) 0.5g
K2HPO4 0.5g Water (水) 1000ml
pH 7.2-7.4
適用範圍:刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)
12. Gause′s Synthetic Agar (高氏合成一號瓊脂)
KNO3 1g Soluble starch(可溶性澱粉) 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar (瓊脂) 20g water (水) 1000ml
pH 7.2-7.4
適用範圍:刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)、白黃鏈黴菌、白色鏈黴菌、抗生鏈黴菌、雙重輪絲鏈黴菌、產色鏈黴菌、燼灰鏈黴菌、天藍色鏈黴菌、滅蚊鏈黴菌、紅黴素鏈黴菌、青色鏈黴菌、球孢鏈黴菌、淺灰鏈黴菌、灰色鏈黴菌、吸水鏈黴菌、淡紫灰鏈黴菌、黃色長孢鏈黴菌、藤黃色鏈黴菌、細黃鏈黴菌、黑化鏈黴菌、玫瑰色鏈黴菌、華美鏈黴菌、嗜熱鏈黴菌、委內瑞拉鏈黴菌、紫色直絲鏈黴菌、紫色鏈黴菌、綠色鏈黴菌
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