Triple Sugar Iron Agar Medium

Formula:

Pancreatic Digest of Casein...10.0
Pancreatic Digest of 
Animal Tissue...................10.0
Sodium Chloride..................5.0 
Lactose...........................10.0
Dextrose...........................1.0
Sucrose..........................10.0
Phenol Red.....................0.025 
Ferrous Ammonium Sulfate.....0.2
Sodium thiosulfate...............0.2
Agar...............................13.0
Final pH 7.3±0.2 at 25℃

Directions:

Suspend 64.5g/L, heat to be boiling for 1 minute, dispensing 16mm × 150mm test tubes, autoclave at 121 ℃for 15 minutes. Bevel and its length is 4-5cm, the length of the bottom is 2-3cm